Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status

Audrey Caro 1, 2, * Arthur Escalas 1 Corinne Bouvier 1, 3 Evelyse Grousset 1 Nicole Lautredou-Audouy 4 Cécile Roques 1 Mireille Charmantier 1 Olivier Gros 2
* Corresponding author
1 ECOLAG - Ecosystèmes lagunaires : organisation biologique et fonctionnement
2 SAE - Systématique, adaptation, évolution
3 TIMONE - Hôpital de la Timone [CHU - APHM]
4 CBS - Centre de Biochimie Structurale [Montpellier]
Abstract : Sphaeroma serratum is a marine isopod species that inhabits seashores from Europe to West Africa. The individuals live under stones in direct contact with reduced sediments and harbour a diverse bacterial community on the cuticle of their pleopods. We investigated the diversity of these epibiotic bacteria on male (pubescent and senescent) and female specimens with electron microscopic obser vations and molecular tools. The microbial community of S. serratum was shown to be composed of at least 5 bacterial morphotypes observed on the pleopodal cuticle in all male specimens. Using fluorescence in situ hybridization, we identified 5 major phylogenetic groups (α-, β-, γ- and δ-Proteobacteria and Archaea) whereas denaturing gradient gel electro - phoresis (DGGE) of PCR-amplified 16S rRNA gene fragments of epibiotic bacteria revealed 50 bands. The bacterial community associated with S. serratum seems more diverse than in other marine crustaceans, such as Rimicaris. The relative diversity of this bacterial community was also studied in relation to the molt cycle. The comparison of DGGE band patterns of several individuals from female, pubescent male and senescent male groups revealed that the bacterial community diversity was dependent on the sex and the age of the individuals and more generally on the molt status.
Keywords : Sphaeroma Molt cycle Epibiotic biofilm DGGE band pattern FISH Crustacean
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Journal articles
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Submitted on : Friday, October 19, 2012 - 8:35:57 PM
Last modification on : Tuesday, May 28, 2019 - 11:02:04 AM

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UNIV-AG | UPMC | SAE | EVOLUTION_PARIS_SEINE | IBPS | EVOL_PARIS_SEINE-BM | ECOLAG | AGROPOLIS | SORBONNE-UNIVERSITE | BS | UNIV-MONTPELLIER | SU-SCIENCES

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Audrey Caro, Arthur Escalas, Corinne Bouvier, Evelyse Grousset, Nicole Lautredou-Audouy, et al.. Epibiotic bacterial community of Sphaeroma serratum (Crustacea, Isopoda): relationship with molt status. Marine Ecology Progress Series, Inter Research, 2012, 457, pp.11-27. ⟨10.3354/meps09711⟩. ⟨hal-00743743⟩

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