Insight of EDX Analysis and EFTEM: Are Spherocrystals Located in Strombidae Digestive Gland Implied in Detoxification of Trace Metals ?

Abstract : Digestive tubules of Strombidae are composed by three cell types: digestive cells, vacuolated cells, and crypt cells. The last one is characterized by the presence of intracellular granules identified as spherocrystals. Such structures are known to occur in basophilic cells of gastropod digestive gland, where they are supposed to be involved in the regulation of some minerals and in detoxification. In this study, energy-dispersive X-ray analysis (EDX) and energy filtered transmission electron microscopy (EFTEM) were used to determine the elemental content of spherocrystals in two Strombidae, Strombus gigas and Strombus pugilis. In freshly collected individuals of both species, the following elements were detected: Ca, Fe, Mg, P, and Zn. Aluminum and Mn were also detected in S. gigas. Their presence in spherocrystals indicates that, in Strombidae, spherocrystals are involved in the regulation of minerals and essential trace metals. In order to answer the question ''are spherocrystals involved in nonessential trace metals scavenging?,'' artificial cadmium and lead exposure by both waterborne and dietary pathways was applied to S. pugilis. No evidence of cadmium (Cd(NO3)2) or lead (Pb(NO3)2) provided by food was found in spherocrystals. Cadmium provided in water (Cd(NO3)2 and CdCl2) causes structural modifications of the digestive gland; however, this element was not trapped in spherocrystals. These results suggest that spherocrystals are not involved in detoxification of such nonessential trace metals.
Type de document :
Article dans une revue
Microscopy Research and Technique, Wiley, 2011, 75 (4), pp.425-432. 〈10.1002/jemt.21073〉
Liste complète des métadonnées

https://hal.univ-antilles.fr/hal-00743762
Contributeur : Pamphile Isch <>
Soumis le : vendredi 19 octobre 2012 - 23:08:12
Dernière modification le : mercredi 18 juillet 2018 - 20:11:27

Lien texte intégral

Identifiants

Citation

Jean-Marie Volland, Jean-Pierre Lechaire, Ghislaine Frebourg, Dalila Aldana Aranda, Gaëlle Ramdine, et al.. Insight of EDX Analysis and EFTEM: Are Spherocrystals Located in Strombidae Digestive Gland Implied in Detoxification of Trace Metals ?. Microscopy Research and Technique, Wiley, 2011, 75 (4), pp.425-432. 〈10.1002/jemt.21073〉. 〈hal-00743762〉

Partager

Métriques

Consultations de la notice

307