An environment affected by anthropogenic activities can play an important role in the spread and concentration of antibiotic resistant bacteria. Under antibiotic selection pressure, exchanges of antibiotic resistance genes (ARG) can occur between bacteria from human, animal and environmental reservoirs. However, these processes are still poorly understood. This work aimed at focussing on the wastewater treatment process, which is a hotspot for selection and emergence of antibiotic resistant bacteria and constitutes a favourable interface for circulation of ARG between bacteria from the different reservoirs. Between 2018 and 2019, extended spectrum β-lactamase producing Enterobacteriaceae (ESBLE) (Enterobacter cloacae complex, Escherichia coli and Klebsiella pneumoniae) were isolated from 266 patients at University Hospital of Pointe-à-Pitre (Guadeloupe). Simultaneously, urban and hospital wastewaters were analysed at different steps of waste treatment and 120 stool samples were collected from animals living near the hospital sewers and the wastewater treatment plant. Enterobacteriaceae were characterised according to their antibiotic resistance profile. Strains with the same antibiotic resistance profile belonging to a particular human-environment-animals continuum were analysed using whole genome sequencing. We isolated a total of 905 ESBLE (292 from humans, 348 from wastewater and 265 from animals). The frequency of ESBLE carriers was important in wildlife and domestic animals living nearby polluted environments (70%). A first genome-based comparison was carried out on 30 ESBL producing E. coli isolated during this study and compared to ESBL producing E. coli collected in farms (n=12). Antibiotic resistance gene blaCTX-M-15 was frequent (17/42). IncFII type was the most common plasmid (15/42). E. coli ST131 was found on the clinical samples only (n=4). One cluster with wastewater and animal isolates differed by ≤ 5 single nucleotide polymorphisms. These first results suggest that animals living near sewers and wastewater treatment plants are subjected to antibiotic resistance pressure and some bacteria and antibiotic resistance genes could be closely related.