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Poster communications

Phylogenetic analyses of Mycobacterium ulcerans from French Guiana using combination of core and accessory genomes

Abstract : In South America, French Guiana is the only Buruli ulcer endemic area, even though the incidence remains low over the years, with a total of 245 cases reported between 1969 and 2013. In a previous study, we reported evidence for uncommon heterogeneity in M. ulcerans (usually characterized by a very low level of genetic variability) and we identified two main contrasted sublineages. Nevertheless, this study was based on classical molecular typing approaches which can sometimes lead to discrepancies in Mycobacterium classification. To overcome these issues, and to move forward in the exploration of M. ulcerans genetic variability, we performed cgMLST (Core Genome Multi Locus Sequence Typing) using whole genome sequences of 25 M. ulcerans strains originated from French Guiana (n=5) and from other countries in the world were Buruli is endemic (n=20). Maximum likelihood phylogeny revealed a total of 5 lineages clearly delineated. As expected lineage L3 previously described by Doig et al. (2012) comprised all isolates from Africa and from Papua New Guinea and, lineage L2 includes Asian isolates from China and Japan. Concerning the lineage L1, Doig et al. initially proposed a clusterization of the M. ulcerans strains Mu_1G897 isolated in French Guiana with MPM isolates (Mycolactone Producing Mycobacteria: M. liflandii, M. pseudoshotsii, M. shinshuense), known to be pathogenic to ectotherms fishes and frogs. In our study, as we included 5 additional M. ulcerans samples from French Guiana that clearly clustered together, we were able to reveal for the first time an independent phylogenetic group called lineage L1.2, while MPM isolates were grouped into L1.1. Finally, the Mexican isolate and the strain Mu_FG102 recently identified in French Guiana constitute the lineage L4; this lineage was also characterized for the first time in this work using WGS.
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https://hal.univ-antilles.fr/hal-02453184
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Submitted on : Thursday, January 23, 2020 - 4:42:26 PM
Last modification on : Friday, January 24, 2020 - 7:42:17 PM

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  • HAL Id : hal-02453184, version 1

Citation

Yann Reynaud, P. Fresia, Gregorio Iraola. Phylogenetic analyses of Mycobacterium ulcerans from French Guiana using combination of core and accessory genomes. Caribbean Science and Innovation Meeting 2019, Oct 2019, Pointe-à-Pitre (Guadeloupe), France. ⟨hal-02453184⟩

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