(Guava (Psidium guajava L.) is a tropical fruit widely consumed around the world. The study was carried out on four selected guava varieties grown in Martinique. Two fruit harvests were gathered at two-year intervals, at the green/yellow color change maturity stage. First, we established the homogeneity of all fruit batches by bootstrapping, ANOVA, box plot and multiple comparison statistical analysis on physical parameters. Red Supreme Ruby clearly differs from the other three varieties, whereas the results for Centeno Prolific are between Beauséjour and Enana Cuba. We determined on puree obtained from a homogeneous batch of each variety the physicochemical and functional properties as well as the antioxidant properties. Our results show that the combination of lycopene and vitamin C, and their synergistic mechanisms in the fruit extracts may be responsible for the high antioxidant activity of guava puree, and we demonstrate a statistically significant positive correlation between EC50 and lycopene.