The aim of this study was first to optimize the extraction and characterization of lycopene, a carotenoid, derived from a tropical fruit, Psidium guajava L., and then to demonstrate its health benefits through its antioxidant power. An extraction procedure was used to reach depletion and a high yield of lycopene, thus minimizing the use of organic solvent. An excellent carotenoid purification was obtained by HPLC-VIS (470 nm) detection in isocracy with a mobile acetonitrile/methanol/tetrahydrofurane phase. The characterization of this pigment has been confirmed by FT analysis. Therefore, we developed an adapted DPPH test to lycopene, which allowed to evaluate its EC50, thus classifying this micronutrient among the good antioxidants. From these results, we can suggest that pink guava (Psidium guajava L.) grown in the West Indies and particularly consumed raw or cooked, contain high levels of lycopene and these results corroborate the health benefit effect of guava and guava-derived products. Reference : Priam F.1, Marcelin O. 1, Marcus R. 1, Jô L-F. 1, Smith-Ravin E-J. Lycopene extraction from Psidium guajava L. and evaluation of its antioxidant properties using a modified DPPH test. IOSR-JESTFT, 11, 2017, 67-73.