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Journal Articles iScience Year : 2022

FtsZ-mediated fission of a cuboid bacterial symbiont

Philipp Weber
  • Function : Author
Gabriela Paredes
  • Function : Author
Tobias Viehboeck
  • Function : Author
Nika Pende
Jean-Marie Volland
  • Function : Author
Michael Vannieuwenhze
  • Function : Author
Jörg Ott
  • Function : Author
Silvia Bulgheresi
  • Function : Author


Less than a handful of cuboid and squared cells have been described in nature, which makes them a rarity. Here, we show how Candidatus Thiosymbion cuboideus, a cube-like gammaproteobacterium, reproduces on the surface of marine free-living nematodes. Immunostaining of symbiont cells with an anti-fimbriae antibody revealed that they are host-polarized, as these appendages exclusively localized at the host-proximal (animal-attached) pole. Moreover, by applying a fluorescently labeled metabolic probe to track new cell wall insertion in vivo, we observed that the host-attached pole started septation before the distal one. Similarly, Ca. T. cuboideus cells immunostained with an anti-FtsZ antibody revealed a proximal-to-distal localization pattern of this tubulin homolog. Although FtsZ has been shown to arrange into squares in synthetically remodeled cuboid cells, here we show that FtsZ may also mediate the division of naturally occurring ones. This implies that, even in natural settings, membrane roundness is not required for FtsZ function.

Dates and versions

hal-03644164 , version 1 (18-04-2022)



Olivier Gros, Philipp Weber, Gabriela Paredes, Tobias Viehboeck, Nika Pende, et al.. FtsZ-mediated fission of a cuboid bacterial symbiont. iScience, 2022, 25 (1), pp.103552. ⟨10.1016/j.isci.2021.103552⟩. ⟨hal-03644164⟩
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