Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment) - Université des Antilles Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2003

Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment)

Olivier Gros
Martine Liberge
  • Function : Author
Abdelaziz Heddi
  • Function : Author
Chaqué Khatchadourian
  • Function : Author
Horst Felbeck
  • Function : Author

Abstract

ABSTRACT Target DNA from the uncultivable Codakia orbicularis endosymbiont was PCR amplified from sea-grass sediment. To confirm that such amplifications originated from intact bacterial cells rather than free DNA, whole-cell hybridization (fluorescence in situ hybridization technique) with the specific probe Symco2 was performed along with experimental infection of aposymbiotic juveniles placed in contact with the same sediment. Taken together, the data demonstrate that the sulfide-oxidizing gill endosymbiont of Codakia orbicularis is present in the environment as a free-living uncultivable form.

Dates and versions

hal-04108669 , version 1 (28-05-2023)

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Olivier Gros, Martine Liberge, Abdelaziz Heddi, Chaqué Khatchadourian, Horst Felbeck. Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat ( Thalassia testudinum Environment). Applied and Environmental Microbiology, 2003, 69 (10), pp.6264-6267. ⟨10.1128/AEM.69.10.6264-6267.2003⟩. ⟨hal-04108669⟩
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